Beta
ManageSearchChatDiscover
64433

BIOCHEMICAL STUDIES ON HEPATITIS C VIRUS INFECTION

Article

Last updated: 24 Dec 2024

OverviewSimilar Items

Subjects

-

Tags

-

Abstract

Hepatitis C virus (HCV) infection is a major worldwide public health problem. The World Health Organization (WHO) estimates that 3% (nearly 170 million people) of the world's population are chronically infected with HCV and that it accounts for around 20% of cases of acute hepatitis and 70% of cases of chronic hepatitis. Detection of HCV antigen in body fluids other than blood is important for assessing possible other routes of viral transmission. This is because body fluids other than blood might be potential sources of HCV infection. Serum and cerebrospinal fluid (CSF) samples of 25 meningitis patients infected with HCV, serum and cord samples from 25 pregnant women infected with HCV in addition, serum and urine samples of 25 patients infected with HCV were included in this study. Also serum, CSF, cord and urine samples were collected from 30 healthy volunteers as negative controls. HCV antigen was detected in these body fluids using ELISA and western blotting techniques. Western blot analysis showing a single immunoreactive band in cord, CSF, urine and serum of HCV infected patients at 27-kDa. ELISA showed high degrees of sensitivity (92%) and specificity (93.3%). The antigen detection method showed high predictive values of positive (97.2%) and negative (82.4%).

DOI

10.21608/blj.2010.64433

Authors

First Name

C. A. Abdel

Last Name

Malak

MiddleName

-

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science Damietta, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

T.

Last Name

Mosa

MiddleName

-

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science Damietta, Mansoura University, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Hawas

MiddleName

-

Affiliation

Biochemistry Division, Chemistry Department, Faculty of Science Damietta, Mansoura University, Egypt

Email

-

City

-

Orcid

-

Volume

6

Article Issue

1

Related Issue

9732

Issue Date

2010-12-01

Receive Date

2010-05-14

Publish Date

2010-12-01

Page Start

94

Page End

105

Print ISSN

1687-4773

Online ISSN

2974-4725

Article File

BLJ_Volume 6_Issue 1_Pages 94-105.pdf

PDF . 377.2kB

Link

https://blj.journals.ekb.eg/article_64433.html

Detail API

https://blj.journals.ekb.eg/service?article_code=64433

Order

7

Type

Original Article

Type Code

988

Publication Type

Journal

Publication Title

Biochemistry Letters

Publication Link

https://blj.journals.ekb.eg/

MainTitle

BIOCHEMICAL STUDIES ON HEPATITIS C VIRUS INFECTION

Details

Type

Article

Created At

22 Jan 2023