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64242

IN VIVO AND IN VITRO EXPRESSIONS OF CCR1 AND CCR5 IN PATIENTS WITH ACTIVE SCHISTOSOMIASIS AND CHRONIC LIVER DISEASE

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Last updated: 04 Jan 2025

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Abstract

The CCL3 receptors, CCR1 and CCR5, are expressed in a series of cell types. The aim of the present study was to demonstrate, in vitro and in vivo, expressions of CCR1 and CCR5 on peripheral blood mononuclear cells (PBMCs) from healthy individuals and Schistosoma mansoni (S. mansoni) infected patients with chronic liver diseases (CLD). PBMCs were isolated from the blood of 55 individuals divided into four groups; Group I: thirty one patients with CLD; group II: six patients with active S. mansoni infection, diagnosed by parasitological examination; group III: eight healthy individuals, non-infected with both schistosomiasis or viral infections, followed by treatment with soluble S. mansoni eggs antigens (SEA) after short term cells culture, for in vitro studies and group IV: ten healthy individuals, non-infected with both schistosomiasis or viral infections and served as a negative control group. All PBMCs from the studied groups were stained with monoclonal antibodies against CD4, CD8, CCR1 and CCR5, then detected by a flow cytometry technique. In-vitro study revealed that CCR1 and CCR5 expressions in SEA treated short term culture of PBMCs were not significantly lower than healthy controls (P=0.56 and 0.298, respectively). Also, In-vivo study, in active schistosomiasis patients, the decrement were not significant (P=0.313 and 0.093, respectively) compared with a healthy control group. While in CLD patients there was an increment in expression of CCR1 with significant value (p=0.014), as well as in CCR5 but the increment were not significant (P=0.099). Patients with active schistosomiasis showed lower CD4 and higher CD8 T-cells count compared with healthy controls. The expressions of CD4 and CD8 T-cells were not significant (P= 0.368, 0.875, respectively). CCR1 and CCR5 expressions may play a role in recruitment of lymphocytes to the CLD patients. CD4 was down regulated and CD8 T cells were up regulated in PBMCs of active schistosomiasis patients.

DOI

10.21608/blj.2010.64242

Keywords

CCR1, CCR5, Schistosoma mansoni, Chronic liver diseases, active schistosomiasis, HCV, SEA, chemokine receptors

Authors

First Name

Samir A.

Last Name

El-Masry

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Affiliation

Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menufyia University, Sadat City, Egypt

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First Name

Mona S.

Last Name

Gouida

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Affiliation

Flow Cytometry and Genetic Units, Mansoura Children Hospital, Mansoura University, Egypt.

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First Name

Khalid M.

Last Name

El-azab

MiddleName

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Affiliation

Medical Analysis Specialist, Egypt.

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First Name

Mahmoud E

Last Name

Nasr

MiddleName

-

Affiliation

Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, Menufyia University, Sadat City, Egypt.

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Volume

7

Article Issue

1

Related Issue

9718

Issue Date

2011-12-01

Receive Date

2010-04-04

Publish Date

2010-12-01

Page Start

45

Page End

68

Print ISSN

1687-4773

Online ISSN

2974-4725

Article File

BLJ_Volume 7_Issue 1_Pages 45-68.pdf

PDF . 509.9kB

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https://blj.journals.ekb.eg/article_64242.html

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https://blj.journals.ekb.eg/service?article_code=64242

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Original Article

Type Code

988

Publication Type

Journal

Publication Title

Biochemistry Letters

Publication Link

https://blj.journals.ekb.eg/

MainTitle

IN VIVO AND IN VITRO EXPRESSIONS OF CCR1 AND CCR5 IN PATIENTS WITH ACTIVE SCHISTOSOMIASIS AND CHRONIC LIVER DISEASE

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Article

Created At

22 Jan 2023