Rheumatoid arthritis (RA) it is a model of multigenic inflammatory disorder in which tumor necrosis factor-α (TNF-α) plays an important role. Genetic factors may be implicated in the susceptibility to disease initiation .Objective: The aim of this study is to elucidate putative association between the −308 G/A polymorphism in the promoter region of the TNF-α gene and 196M/R polymorphism of TNFRII genes and susceptibility to disease onset of RA. Methods: A total of 160 RA patients and a control group of 40 healthy subjects were available for the study. All patients fulfilled the American College of Rheumatology revised criteria for RA, Single-nucleotide polymorphisms (SNPs) at position −308 of TNF-α and 196 of TNFRII genes were determined using restriction fragment length polymorphism–polymerase chain reaction (PCR–RFLP). The A allele of the −308 promoter polymorphism was more prevalent among the patients. 308GA genotype of TNF-α is associated with a significantly higher serum TNF-α level. TNFRII 196R allele more prevalent among the patients .Our findings suggest that the A allele of TNF-α and TNFRII 196M/R polymorphism are associated with RA susceptibility.