Bone marrow is considered one of the most widely used sources for obtaining stem cells. The ease of techniques, absence of ethical dilemmas makes it one favorable source for regenerative medicine trials. In the present study we isolated MSCs from the bone marrow of Sprague Dawley rats. Characterization of the isolated cells revealed their positive CD29, 90 (Mesenchymal stem cells) and negative CD 45 denoting their mesenchymal lineage and the supporting the possibility of isolating MSCs from bone marrow. As such, this would invite us to use BMSCs for further preclinical and clinical trials in the area of regenerative medicine.