Background: Wound healing is a complicated process of skin repair after injury. This process requires a coordinated interplay among cells, growth factors, and extracellular matrix proteins. Central to this process is the endogenous mesenchymal stem cell (MSC), which coordinates the repair response by recruiting other host cells and secreting growth factors and matrix proteins. MSCs are self-renewing multipotent stem cells that can differentiate into various lineages of mesenchymal origin such as bone, cartilage, tendon, and fat. In addition to multilineage differentiation capacity, MSCs regulate immune response and inflammation and possess powerful tissue protective and reparative mechanisms, making these cells attractive for treatment of different diseases. Aim: The beneficial effect of exogenous bone marrow-derived mesenchymal stem cell (BM-MSCs) on wound healing was observed but the mechanisms involved are barely understood. In this study, we examined signaling factors released by BM-MSCs and their effects on the cells participating in wound healing. Materials &Methods: Thirty female mice were randomly divided into three groups and these female mice were anesthetized and 6-mm full thickness, 4-cm wide deep wounds were made , then the formed colonies of the 2nd generation of BM-MSCs were injected intraperitoneally in each mice in the second group while intraregionally in the third group. These mice observed till complete healing then reverse transcription polymerase chain reaction (RT-PCR) was done for ICAM-1,ITG-β1,ITG-β2. Results: This study found that MB-MSCs promote the wound healing process through secretion of wound healing- related proteins and a broad spectrum of cytokines and growth factors, particularly vascular endothelial growth factor (VEGF).