Background: Kidney stones are one of the most painful urologic disorders. Scientists have found evidence of kidney stones in a 7,000-year-old Egyptian mummy as they may contain various combinations of elements. Zamzam water showed highly significant readings in some inorganic elements, including Na, Ca, Mg, K, HCO3, Cl, Fl, NO3, and SO4. The levels of these elements in Zamzam water may play a critical role in its effectives in the inhibition of calcium oxalate formation. Aim: This study was carried to investigate the effect of Zamzam water, and treated tab water with Ca, Mg, F, Cl and Na in adult male albino rats suffer from kidney stones induced by ethylene glycol. Materials & Methods: Kidney Calcium oxalate stones were induced in animals under study by oral administration of 0.75% of ethylene glycol dissolved in drinking water. Fifty rats were divided into equal 5 groups; each group was containing 10 rats: negative control, positive control (drinking tab water contains 0.75% ethylene glycol); negative Zamzam group (drinking Zamzam water only); Zamzam treated group (drinking Zamzam water contains 0.75% of ethylene glycol); and the last group tab water treated group (drinking treated water contain 0.75% ethylene glycol). After 4 weeks of treatment urine analysis and some biochemical analysis, were carried out in all studied groups. Results: we found that; significant differences in urine, Kidney functions and liver functions analysis in positive control group compared to negative control, while Zamzam group showed non-significance. Whereas Zamzam treated, showed a significant difference in all parameters compared to positive control group, more than tap water treated group. Conclusion: Zamzam water and treated tap water may play a critical role in its effectives in the inhibition of calcium oxalate formation.