Background: Obesity has been on the increase and is now an international health problem thus early must be control and prevent childhood obesity. Objective: This work was conducted to investigate the biochemical effect of date seed extract on lipid profile and hepatic antioxidant status in experimentally obese rats. Results: In this study 112 male albino Wister rats were involved and were divided randomly into fourth groups (28 rats in each group) and treated as following for 10 weeks: Group 1 served as control and received basal diet Group –II (Positive control obese group) rats were fed on high fat diet, Group III (date seed extract group): rats were fed on basal diet with administered orally date seed extract daily in a dose of 2 ml / kg b. wt. for 10 weeks Group IV rats were fed on high fat diet for 4 weeks followed by administration of date seed extract orally in a dose of 2 ml / kg b. wt. daily for 6 weeks. beside continuation feeding on HFD. The results showed that the daily oral administration of pits of date palm caused decrease in total cholesterol, LDL-cholesterol, VLDL-cholesterol, triglycerides levels, atherogenic indices, glucose and MDA level while caused an increase in HDL-cholesterol, reduced glutathione and superoxide dismutase activity. Concerning liver histopathology, it was found that oral administration of date seed extract decrease fat droplet gradually and retain liver to normal condition. Conclusion: On conclusion the date seeds have the potential to improve serum biochemical values, antioxidant status and normalized hepatic tissue in obese male rats.