Nutritional studies recommend the regular consumption of fruits and vegetables to favor a healthy quality of life. This study was carried out to evaluate the efficacy of hesperidin and tiger nut against the carcinogenic activity of DMBA in female rats. 72 adult Sprague Dawley female rats were divided equally into six groups: control group (I); hesperidin (HES) treated group (II); tiger nut (TN) treated group (III); DMBA treated group (IV); HES-DMBA treated group (V); and TN-DMBA treated group (VI). There was a significant increase in the levels of serum total sialic acid (TSA), progesterone (Prog) and estradiol (E2), and significant decreases of body weights and reduced glutathione (GSH) level and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities of DMBA treated group compared with the control. Histopathological observations of mammary gland showed that only female rats of DMBA and TN-DMBA treated groups showed breast cancer, while there was no evidence of malignancy in HES-DMBA treated group. In conclusion, our results suggested that supplementation of a diet with hesperidin provided antioxidant defense with chemoprotective activity more significant than tiger nut against the toxicity of DMBA in breast tissue.