Bachground: Methlenetetrahydrofolate reductase (MTHFR) is one of the most important enzymes for folate metabolism. This enzyme is mapped on chromosome 1, The methylene tetra hydro folate reductase (MTHFR) gene is a polymorphic gene involved in folate metabolism, DNA biosynthesis, methylation and genomic integrity in actively dividing cells. The MTHFR A1298C polymorphism is likely to play an important role in the susceptibility to breast cancer. Objectives: In this case-control study, we examin the role of MTHFR A1298C polymorphism in breast cancer patients. Methods: We examined the MTHFR A1298C polymorphism in 47 women with breast cancer and 42 healthy women using PCR- RFLP method. Results: The alleles frequency of the MTHFR A1298C were 41.5% and 58.5% for A and C alleles in the breast cancer cases and 47.6% and 52.4% in the controls respectively. The genotypes frequency of the MTHFR A1298C for AA , CC and AC in breast cancer patients were 14.9% , 31.9% and 53.2% and in control subjects were 9.5%,14.3% and 76.2% respectively . There is increased risk for breast cancer under 3 models (Allellic C vs.A) ,Homozygote ( CC vs.AA) and Recessive(CC vs.AC+AA) [OR 1.282,1.429 and 2.813 respectively] . conclusion: our data suggest that the MTHFR A1298C alleles and genotypes may play arole in breast cancer susceptibility but not astrong risk factor for women with breast cancer.  Conclusions: Our findings suggest a possible association between IL-10(-1082 G/A) promoter polymorphism and HCV infection, which may confer a higher risk for developing HCV infection.