Background: pyrazolines are used widely in the current decades due to their various biological and pharmacological activities Aim: The main objective of the present study is to evaluate the chemotherapeutic effects of some novel substituted pyrazoline compounds against animal carcinogesis. Materials & Methods: the synthesized Pyrazoline derivatives were characterized by IR spectroscopy, and then their effects on Ehrlich Carcinoma were studied by evaluation their antitumor activity (viability of tumor cells, and life span prolongation), and estimation of antioxidants (catalase, superoxide dismutase, glutathione peroxidase activities, glutathione reduced content, and total antioxidant capacity). Results: Pyrazoline derivatives showed a significant reduction in the volume and count of Ehrlich cells. Also, these compounds suggested potential antioxidant activity by elevation the catalase, superoxide dismutase, glutathione peroxidase activities, and glutathione reduced content. Conclusion: the synthesized compounds have potent antitumor, antioxidant, and decrease the survival of cancer cells.