Background: Silver nanoparticles (AgNPs) was recommended in medicine and veterinary medicine fields, and can be used as drugs for treatment of some diseases. Aim: the toxicity of nanosilver is not well known, it is essential to examine its safety. The experimental albino rats were divided into five groups , each group comprising of ten rats, after adaptation in the animal houses in Faulty of Vet. Medicine Suez Cana University for one week. The groups named as N1, 2, 3, 4 and 5. Group N1, was called control group and was gives deionized water. Blood samples were collected at the end of experiment; serum samples were separated for determination the values of IL-Lβ, IL-2, IL-6, IL-10 and TNF-α. Ag-NPs administration showed a variable significant increase in the levels of Il-Lβ, IL-2, IL-6, IL10 and TNF- α . Conclusion : Nanoparticles induce inflammatory immune responses at lower concentrations and chemokines are the major cytokines induced at early stage of exposure to silver nanoparticles..