Background: Silver nanoparticles (AgNPs) was recommended as a disinfectant and can be used as drug in the treatment of some non- curable disease in lives stock. Aim: the toxicity of nanosilver is not well known, it is essential to examine its safety. Material and methods: fifty male rats were used to assess the dose – dependent manner effects of AgNPs. Adult male rats were divided randomly into five groups, in the four experimental groups, nanosilver particles were injected intrapretoneally for 30 consecutive days at doses of 0.25, 0.50, 1.0 and 2.0 mg/kg 1 B.W. Rats in control group received equal injections with deionized water. Liver sample were collected and stored in liquid nitrogen tank for estimation of some gene expressions. Results: AgNPs administration showed a significant increase in the gene expressions of TNF-α, P53 and caspase -3 levels conclusions: It was concluded that injection of AgNPs in high doses for long period produced changes in gene expression and apoptotic effects were indicated