Background: Titanium dioxide nanoparticles (TiO2 NPs) were healthy well-known as a photocatalyst and it was extensively utilized for photocatalysed corrosion, sensor essentials, water distillation and had numerous other submission. Also, in food industry. However they also had poisonous result on animals uncovered to them. Objectives: we examined the probable ameliorative result for the two flavonoids morin and rutin which had apotent antioxidant activity, against TiO2 nanoparticles-induced toxicities in rat liver. Materials and methods: Albino rats (n =70, weight 150-200g) were divided into seven groups. Group (G1) (control), G2 (treated with morin 30 mg/kg), G3 (treated with rutin 80 mg/kg), G4 (toxic group that administrated orally with titanium dioxide nanoparticles 100 mg/kg), G5 (TiO2 NPs+ morin), G6 (TiO2 NPs+ rutin), G7 (TiO2 NPs+ morin+ rutin). The evaluated parameters included liver function markers, some minerals and electrolytes, antioxidant (superoxide dismutase SOD, catalase CAT and glutathione GSH) and oxidative stress malondialdehyde (MDA) biomarkers in addition to DNA fragmentation and histopathological examination of liver tissues. Results: These experimental study showed that a significant increase of  serum ALT, AST, ALP activities. Also, serum potassium (K) and phosphorous (P) levels, hepatic MDA content, comet, tail length, DNA in tail and tail moment of DNA fragmentation were detected in TiO2 NPs (G4) group indicating liver damage which compared with control group (G1) but  this parameters decreased significantly in morin (G5) or rutin (G6) when compared with toxic group (G4), in combination group (TiO2 NPs+ morin+ rutin) (G7) succeeded to decrease significantly in this parameters and returns to (G1). Also, our results showed that a significant decrease in total protein, albumin, sodium (Na), calcium (Ca) levels, glutathione (GSH) level, superoxide dismutase (SOD) and catalase (CAT) activities in toxic groups (G4) but succeeded to increase significantly in this parameters in G5, G6 and G7 in compared with control group and restore it. Conclusion: TiO2NPs oral administration induced toxic effects and DNA damage in the liver that may be attributable to oxidative stress.Also, administration of morin and/or rutin with TiO2NPs offers protection against their damaging effect.