Liver biopsy is invasive and prone to complications but it stills the gold standard for assessing hepatitis C virus (HCV)-related fibrosis. This study was designed to study non-invasive methods for the assessment of liver fibrosis. Our aim was to validate and compare the performance of MMP-8, upA in addition to GAL-3 as simple blood markers for different stages of liver fibrosis in HCV patients. The study was carried out on 90 individuals divided into the following groups: group 1: normal control, group 2: F1 = portal fibrosis without septa, group 3: F2 = few septa, group 4: F3 = numerous septa without cirrhosis and group 5: F4 = cirrhosis. Our results showed a positive correlation between MMP-8, upA and GAL-3 level and degree of liver fibrosis. We can conclude that, using of MMP-8, upA in addition to GAL-3 as simple biochemical markers for the assessment of hepatic fibrosis as alternative to liver biopsy.