Background: Hydrogen peroxide is a key component of fungal biomass degradation. Fungal extracellular enzymes can produce this reactive oxygen species or depend on it as a co-substrate. The measurement of H2O2 in proximity of enzymes bound to biomass is important to elucidate its production rate by oxidases, its consumption rate by peroxidases. Objectives This work reports the fabrication and characterization of pencil graphite electrode (PGE) and its application to measure the H2O2 production by cellobiose dehydrogenase (CDH). Methods: The limit of detection (LOD) and the sensitivity of pencil graphite electrode were detected for both of bare and modified PGE, respectively. The H2O2 generation of cellulose immobilized CDH was measured using the MOF modified PGE. CDH produces H2O2 in the presence of cellobiose as a substrate. Results: MOF modified PGE exhibited low LODs (571 μM) and high sensitivities (2.35μA mM-1mm-1). The lower acquisition costs and higher simplicity compensate for the higher LODs and lower sensitivities when compared to platinum electrodes. Conclusions: PGE was easy to fabricate, multiply usable and cost effective which make it a strong and promising tool for H2O2 measuring.