Aim : Assess the level of Annexin-1 and explain the relationship between ANX-1 level and periodontal parameters. Subjects and methods: 40 female patients (20 pregnant and 20 non-pregnant) were classified into four groups. Group I: 10 pregnant female patients suffering from gingivitis. Group II: 10 pregnant female patients suffering from periodontitis. Group III: 10 non-pregnant female patients suffering from gingivitis. Group IV: 10 non-pregnant female patients suffering from periodontitis. All patients were received conventional periodontal therapy and evaluated clinically at baseline, 4 and 12 Ws. The laboratory evaluation of ANX-1 level was done at baseline, 4 and 12 Ws. The data were collected, tabulated, and statically analyzed by SPSS (Statistical Package for Social Science). Results: The clinical parameters plaque index, gingival index, probing depth, and clinical attachment level were recorded at baseline, 4 and 12 Ws. In all groups, statistically significant differences were shown in four groups at different intervals when compared to baseline. Plaque index between the four groups representing no statistically significant difference at different intervals. Gingival index shows a statistically significant difference in pregnant and non-pregnant groups at different intervals. Probing depth shows a statistically significant difference in group I when compared with other groups at different intervals. Clinical attachment level shows a statistically significant between pregnant groups and non-pregnant groups at different intervals. ANX-1 level shows a statistically significant difference in group I when compared with other groups at baseline. Conclusions: ANX-1 in GCF showed marked elevated levels with significant differences during pregnancy with gingivitis