Aim: it is to evaluate the effect of dentine desensitizing agents on shear bond strength of two totals-etch adhesive systems. Subjects and methods: Sixty human premolars free from caries or cracks were used in the study and randomly divided into six groups, group1; Adper Single bond 2, group 2; Prime & Bond NT, Group3; Adper Single Bond and Gluma desensitizing agent, group4,Adper Single Bond2 and Vivasens desensitizer, group5;Prime& Bond NT and Gluma desensitizer, group 6; Prime& Bond and Vivasens, then Z250 XT nanohybrid composite resin was packed in the mould and cured, then shear bond strength was measured by INSRTON universal testing machine, One-way ANOVA and Turhey's tests were used for statistical analysis (P value ≤ 0.05). Results: group 3 Adper single bond2 and Gluma registered the highest mean shear bond strength value (12.806 ± 0.89), followed by group 1: Adper Single bond2 registered (12.046 ± 0.84), group 2; Prime & Bond NT recorded (11.21 ±0.47), group 4, Adper Single bond2 and Gluma registered (11.1435 ± 0.54), group 5, Prime & Bond and Gluma registered (11.818 ± 0.56), and group 6, Primr & Bond and Vivasens recorded (11.0485 ± 0.54). Conclusion: Gluma improved shear bond strength with both tested total-etch adhesive systems