Aim: It was to evaluate the use of topical ozone gel on post-operative sequelae following impacted lower third molar surgery. Subjects and Methods: the current study included 30 patients divided randomly into equal three groups. Clinical examination included assessment of maximal mouth opening and cheek dimensions preoperatively. Panoramic x-ray was used for evaluation of the location and configuration of impacted lower third molar, surrounding bone, mandibular canal and adjacent tooth. The maximal mouth opening and check dimensions were remeasured postoperatively at days 2, 5 and 7. All readings were recorded and analyzed statistically. Results: Results showed statistical significance regarding pain while there was no statistical significance regarding mouth opening and swelling. Conclusion: Topical ozone gel is useful for the reduction of postoperative pain after lower third molar surgery.