Nowadays, many herbicides are used for pest resistance purposes. One of these is glyphosate. Although, the glyphosate is a broad-spectrum herbicide, it may be toxic to humans. The purpose of the present study was to determine the glyphosate-induced histopathological changes in the parotid salivary gland, tongue, and palate after subchronic exposure of rats to glyphosate. The present investigation was carried out on twenty healthy adult male Sprague-Dawely rats. The rats were exposed to single dose of the glyphosate (15 mg/kg body weight) for the period of 2 weeks which was the duration of the study. At the end of the experiment, the rats were sacrificed and sections of the parotid salivary glands, tongue and palatal mucosa were taken for histopathological preparation and examination. The results demonstrated that, in the experimental glyphosate treated group, there are remarkable changes in the tongue, palate or the parotid salivary gland. It was concluded that glyphosate may had adverse effect on tongue, palate and parotid salivary glands.