Exposure to anesthetic gases, particularly halogenated agents, can result in health hazards on operating room personnel especially anesthesiologists. This study aimed to evaluate the effect of duration of exposure to anesthetic gases on the liver and kidney functions of anesthesiologists. 80 blood donors participated in the study; 20 healthy unexposed office workers (control group), and 60 exposed anesthesiologists. Exposed subjects were divided into 3 groups; group I exposed to anesthetic gases for < 10 years, group II exposed to anesthetic gases for 10-20 years, while group III has >20 years of exposure. During work, all exposed subjects were exposed to a complex mixture of anesthetic agents. The most commonly used anesthetics were halothane and Isoflurane. Venous blood samples were drawn from each subject into tubes without addition of anticoagulant. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), Albumin, Total protein, Blood Urea Nitrogen (BUN), creatinine (Cr) were analyzed. ALT and AST showed significant difference between groups while their values were within normal ranges. Concerning the kidney function tests among anesthetist exposed to anesthetic agents, BUN and creatinine levels were within normal range without significant difference between groups. In conclusion, the current study showed that anesthesiologists were more at risk to develop health hazards related to exposure to waste anesthetic gases.