Silver nanoparticles (AgNPs) are widely involved in the medical products owing to their antibacterial activity. However, their extensive use causes human hazards via dermal, inhalational and parenteral routes. The aim of the current study was to investigate the toxic effect of different doses of AgNPs on the ovaries and the kidneys of the adult female Albino Rats. Forty female rats were randomly allocated to three treated groups and a control to assess the toxic effect of AgNPs injected intraperitoneally at 1, 2, 4 mg/kg respectively over a period of 28 days. Renal and ovarian tissues together with blood samples were collected and examined for various biochemicals, hormonal and histopathological changes. Oxidative stress biomarkers were also measured. The results revealed a decrease in Luteinizing hormone (LH) and Follicle-stimulating hormone (FSH) in addition to increased levels of Blood Urea Nitrogen (BUN) and serum creatinine among the studied groups in comparison to the control. Furthermore, there were considerable generations of reactive oxygen species (ROS) expressed by elevated plasma malonaldehyde (MDA) level and decreased antioxidant glutathione peroxidase (GPx) and antioxidants-superoxide dismutase (SOD) levels together with demonstration of histopathological alterations in the renal and ovarian tissues in the three studied groups compared to the control group. Conclusively, renal and ovarian tissues displayed both functional and histopathological structural alteration following exposure to AgNPs in a characteristic dose-dependent manner. This can be partly explained by generation of ROS indicated by elevation of MDA level and reduction of GPx and SOD levels.