Human identification is one of the most challenging sciences. Study of lip prints is an interesting method for identification in forensic medicine. The aim of the present work is to determine the pattern of lip prints and evaluate its uniqueness in a sample of Egyptian population. The study included 955 subjects (2-65 years old). Lip prints were taken by direct rolling methods against hard background and each lip print was divided into six areas to be examined by magnifying hand lens. Thereafter, they were scanned and examined by the microsoft office picture manager program. The results revealed that the lip print pattern for each individual was unique and the complete vertical type was the commonest among males and females. Sex could not be differentiated from the lip pattern. It can be concluded that lip print analysis is an important tool for personal identification. It is recommended to establish a database for all individuals in a certain locality, hoping to be a reference in both civil and criminal cases.