Electronic cigarettes (e-cigarettes) are increasingly used worldwide even though, only rare information is available on their health effects. Hence, the electronic cigarette smoking effects were examined in comparison to the conventional reference cigarette (tobacco cigarettes) using the commercially available cigarettes (Cleopatra), on a fixed puffing regimen based on a subchronic smoke exposure study. A ninety day-exposure study in rats showed marked anemia, with reduced RBCs, WBCs, and platelets count with electronic cigarette smoke. The smoke related clinical pathology changes, revealed disturbed hepatic and renal functions with the electronic cigarettes. The changes included also hypercholesterolemia, acidosis and hyperkalemia, together  with hyponatremia and reduced total serum Ca level with the electronic cigarettes. The histopathological findings were of a more pronounced derangement in the lower respiratory tract in rats exposed to electronic cigarette smoke. The hepatic and renal histopathological findings revealed more hemorrhage and degeneration with electronic cigarette smoke. Collectively, the biological effects seen for the smoke of electronically heated cigarettes and conventional cigarettes were comparable with more pronounced toxicological health hazards following subchronic exposure to electronic heated cigarette smoke.