Pesticides are often used indiscriminately in large amounts causing environmental pollution. Pesticides cause adverse effects on different body systems, including hematological and endocrine systems. The ability of pesticides to disturb thyroid function remains unclear. So, the present work studied the possible correlation between chronic some pesticides exposure, used at least within the past 12 months, such as phosphoro di thionate (PDT) and dichloro-diphenyl trichloroethane (DDT) and disturbance in both thyroid hormones levels and some biochemical markers. The study was conducted from the 1st of December 2016 to 1st of July 2017. It involved 100 cases who were recruited from general internal medicine of Al-Azhar and Mansoura University Hospitals, in addition to 50 subjects as a control group from medical students. After free informed consent, 10 mL of blood were separated and the serum was divided into three aliquots, one of which for residues extraction of (PDT) and (DDT) by using high performance liquid chromatography (HPLC) and two of which were stored at -8 Cº for routine laboratory investigation including thyroid hormones levels. Cases and controls were subjected to a standardized questionnaire. Then clinical examination to all groups was done stressing on manifestation of thyroid disease. In general a disturbance in thyroid hormones levels (hypothyroidism) in the present study, revealed a strong correlation between it and working or exposures to PDT (Malathion) as it is positive in (46.0%) and (DDT) as it is positive in (55.0%), especially among agriculture working in rural area. There is a significant difference between cases and control groups as regard; serum creatinine, serum cholesterol, high-density lipoproteins, low-density lipoproteins and thyroid hormones. There is also significant positive correlation between pesticide exposures and TSH level. It is recommended that any patients with suspect a manifestation of hypothyroidism must be screened for pesticides especially in rural areas dealing with them.