Background: Diabetes mellitus is one of the most common metabolic disorders that disturb the health of mothers during pregnancy and their babies. MCP-1 is a chemokine which accountable for the calling of inflammatory cell. It was linked to diminished cardiac function. HbA1c level is also considered a marker of follow-up and treatment in diabetes mellitus. Objectives: The study intended to measure the levels of cord blood Hemoglobin A1c and MCP-1 and correlate their levels with echocardiographic parameters in infants of diabetic mothers (IDMs). Patients and methods: it is a descriptive cross-sectional comparative one which comprised 110 term neonates separated into two groups, the first group included 70 neonates born to diabetic mothers (IDMs) and 40   healthy neonates born to healthy non-diabetic mothers as a control group. This study was conducted from April 2016 to April 2017 at the Maternal and Children Hospital of El Minia.  During the first week of life, all babies were evaluated for medical history, thorough clinical examination with a focus on cardiac examination, and laboratory investigations which included CBC and neonatal blood sugar as routine investigations& HbA1c and MCP-1 as specific investigations in addition to echocardiographic assessment. Results: IDMs have increased cardiac dimensions, impaired diastolic function, and lower fractional shortening compared to the control group. Cord MCP-1and HbA1c was significantly high in IDMs and their levels significantly correlated with the echocardiographic parameters in IDMs the sensitivity and specificity of HbA1c in detecting cardiomyopathy was 83.3% and 95% with positive and negative predictive value 83.7 and 88.1 awhile for MCP-1 sensitivity and specificity93.3% and 97.5% positive and negative predictive values were93.1 %and 95.1%. Conclusion:  MCP-1 and HbA1c were found to be excellent predictors for cardiomyopathy in infants of diabetic mothers with gestational diabetes.