Obesity is a multi-factor chronic condition caused by a amalgamation of genetics, environment, and an energy intake/expension imbalance. Modern civilization is seeing a significant growth in obesity, as well as an increased risk of metabolic syndromes in the general population due to lifestyle change. Even though obesity is a medical problem, maintaining individual's quality of life will require weight control. Weight loss goals is to reduce health risks, promote weight loss, and prevent weight gain . Weight management herbs include thunder god vine (roots).. Herbs like thunder god vine (roots) are used for weight management. Various laboratory studies proved that using thunder god vine extract might causes damage to multiple organs and tissues, and is fatal in some cases. The goal of this study is to measure toxicity of two doses of thunder god vine root extract, low 5mg/kg and high 7.5mg/kg, on the liver, kidney, and testis tissues of fat diet-induced obese male rats.. Histological examination of the obtained sections of all animal groups revealed that treatment with two different doses of the extract in obese rats showed no changes in the seminiferous tubules and different stages of spermatogenesis in testis and. Liver injury and nephrotoxic effects were reported after treatment with the plant extract at with two doses.