Foodborne diseases and poisoning are the widespread in the world. It is a potential threat in human health. This study aimed to investigate alternative antibacterial compounds from actinomycetes isolated from medicinal plant in Sinai, Egypt, against multidrug resistant foodborne pathogens (Salmonella spp and Staphylococcus aureus ). Bacteria isolated from meat. In the present study a total number of 100 random meat samples were collected from Port-Said governorate. Out of which, 16% were found contaminated with S.aureus while 7% were found contaminated with Salmonella. Susceptibility of the isolates to 12 antibiotics was performed using the Kirby-Bauer modified disc diffusion technique .All the S.aureus and Salmonella isolates were found resistant to at least two antibiotics. About 100 % of S.aureus isolates were resistant to Ceftriaxon , 81.25% were resistant for Ampcillin , 75% were resistant to Oxytetracycline , while 100 % of Salmonella isolates were resistant to Erythromycin , 85.7%were resistant for Rifampicin. Two of the S.aureus isolates showed multidrug resistant to 6 antibiotics from 12 antibiotics tested. One of the Salmonella isolate was also found resistant to 5 antibiotics from 12 antibiotics tested. A total 41 extracts from endophytic actinomycetes were screened for antimicrobial activity against multidrug resistant S.aureus and Salmonella. Out of 41 actinomycetes extracts 11 were showed high antibacterial activity against the isolated foodborne pathogens (S.aureus and Salmonella). Results of the present study have shown that the antimicrobial compound derived from extracts of the endophytic actinomycetes may be useful in developing antibiotics against multidrug-resistant bacteria.