The current work aims to study the bioaccumulation of aluminum (Al) ions in the tissues of male rats, as well as to shed light on the relationship of that accumulation to the ionic content of some elements in these tissues. To achieve this goal, the rats were divided into two groups, the first group (control) was intranasal instilled with deionized water, whereas the second was given a single acute dose of nanoalumina (LD25 at 96h that's equivalent to 1.66 g /kg b. wt.). After 24 hours, compared to the control group, the bioaccumulation of Al ions in the liver, kidneys, spleen, lungs, and brain had a significant increase. The bioaccumulation of Al ions in all tissues was associated with a significant decrease in the ionic content of iron, zinc, and copper. On the contrary, the ionic content of calcium was increased. The Al accumulated in most tissues has exhibited an inverse significant relationship with the levels of Fe, Zn, and Cu but the Ca ions showed a positive relationship. In conclusion, the bioaccumulation of Al ions caused a significant effect on the most tissues ionic content of Fe, Zn, Cu, and Ca in male rats.