Background: Infection of hepatitis B virus (HBV) is foremost communal health disquiet around the world. In this sense, Egypt has a seroprevalence of HBV that is somewhere in the middle. There are ten genotypes of HBV (A–J), each with its own geographical spread. Africa is one of the supreme endemic areas, by five genotypes (A-E) were discovered for HBV. Genotype D is the most dominant in the Middle East. There is a scarcity of information about HBV genotyping in Egyptians. Objective: The goal of this research was to find out the genotypes of HBV that were most prevalent among Egyptian patients. Patients and methods: This study included 190 serum samples from patients at the Hospital of Al-Hussein at Al-Azhar University in Cairo, Egypt, whose hepatitis B surface antigen (HBsAg) test result was positive. Of the 190 serum samples, HBV DNA was detected in only one hundred (100) serum samples, and they were the only ones comprised in the research. Patients were sectioned into two collections: 40 from Upper Egypt governorates and 60 from Lower Egypt governorates. The INNO-LiPA technique, which is predicated on the notion of reverse hybridization, was used to determine HBV genotypes. Results: Genotype C represented for 60% of all infections and was mostly distributed among patients in Lower Egypt's governorates, while genotype D represents for 40% of all infections and was mostly spread among patients in Upper Egypt's governorates. Conclusion: These findings substantiated that the most dominant genotype in Lower Egypt governorates was genotype C, while the most dominant genotype in Upper Egypt governorates was genotype D.