Background: Risk of development of cardiovascular disease (CVD), especially coronary artery disease (CAD) is increased in patients with connective tissue diseases (CTD). Data from previous studies showed double fold elevation in the risk of development of heart failure (HF) in CTD patients. Objective: To assess left ventricular (LV) and right ventricular (RV) systolic functions in patients with CTD by 2D speckle tracking Echocardiography (STE). Patients and Methods: This was a case-control study conducted on 120 subjects (100 connective tissue disease cases and 20 healthy controls) to assess LV systolic function and RV systolic function in connective tissue disease patients by 2D speckle tracking Echocardiography. Patients were classified according to presence of connective tissue diseases into 2 independent groups: Group A: connective tissue diseases patients (100 patients), and Group B: (20 healthy controls). All patients were recruited from the outpatient clinic in Al-Hussen University Hospital between September 2020 and April 2021. Results: As regard Echocardiographic LV parameters of the studied groups, we found that there were significant differences between the groups regarding global longitudinal strain (GLS). (More negative GLS in CTDs patients) and mitral annular plane systolic excursion (MAPSE) (P<0.001). RV Echocardiography shows a significant difference between the groups regarding right ventricular end diastolic dimension (RVEDD), right ventricular end systolic dimension (RVESD), GLS (more negative GLS in CTDs patients) and tricusped annular plane systolic excursion (TAPSE) (P<0.001). There was a positive correlation between RV-GLS with TAPSE, while there was a negative correlation between RV-GLS with RVEDD and RVESD. Conclusion: The GLS parameters in 2D speckle tracking echocardiography were a noninvasive cost-effective tool with a desirable diagnostic value in patients with CTD. GLS impairment was common in 2D speckle tracking echocardiography findings in our patients with CTD and this was statistically significant, denoting early systolic function. Longer durations and high disease activity index significantly effect of GLS. GLS is an excellent noninvasive tool for early detection of subclinical LV and RV systolic dysfunction in patients with CTD.