Background: Corneal endothelium is responsible for maintaining corneal transparency throughout life by pumping excess fluid out of the stroma, and keeping stroma in its usual dehydrated state. It makes the measurement of central corneal thickness (MCD), cell size (CV) in the cell size, hexagonal appearance of the central corneal thickness (CCT). These parameters provide an index of the functional status of corneal endothelial layer. Myopia is a common pathologic change of the eye. Objective: To evaluate the corneal endothelial cell changes in myopic patients by indirect specular microscope. Patients and methods: A prospective controlled study was carried out from June 2020 to February 2021 at Al-Hussein University Hospital, and a total of 120 eyes of myopic subjects aged 20-40 years old, 40 eyes were low myopia < -3.00 diobter, 40 eyes were moderate myopia from (-3.00) to (-6.00), and 40 eyes of high myopic subjects > -6.00 diopters sphere were recruited. Results: Our results showed that corneal endothelial cell layer in high myopic eyes tended to have less endothelial cell density and cell hexagonality compared to moderate and low myopic eyes. Significance difference. Nevertheless, there was no significant difference in CCT between low and moderate myopic eyes. Also no significance difference in hexagonal cell percentage between high and moderate myopic eyes. Conclusion: Myopia is a factor that affect the corneal endothelial cell density and morphology.