Background: Ovarian cysts are common in reproductive-aged women. Most cysts are normal functional cysts that typically resolve spontaneously and do not require treatment. However, pathologic cysts such as endometriomas and dermoids may require surgical intervention. Objective: To evaluate the impact of laparoscopic ovarian cystectomy on ovarian reserve regarding ovarian volume, antral follicle count (AFC) and ovarian doppler velocimetry. Patients and Methods: A prospective observational study was conducted at 15 May Specializes Hospital on 80 women, less than 35 years old, with single unilateral ovarian cyst (endometriotic or non-endometriotic) ovarian cysts. Presence of ovarian cyst was diagnosed by preoperative transvaginal ultrasound (TVUS) scan which was confirmed at laparoscopy. All laparoscopic ovarian cystectomies were performed under general anesthesia. Results: There was a statistically significant decrease in ovarian volume (OV) volume and AFC in operated side than contralateral side while no statistically significant difference found between them regarding resistive index (RI). Conclusions  Laparoscopic ovarian cystectomies were risk factors for decreased ovarian reserve and may impair the future fertility.