Background: Upper gastrointestinal (GIT) bleeding remains one of the most important emergencies. It has been widely accepted that the first-line treatment for acute upper GIT bleeding, especially peptic ulcer bleeding, is endoscopic hemostasis. Endotherapy for upper gastrointestinal bleeding can be challenging. Hemospray is a novel hemostatic agent for the treatment of upper gastrointestinal bleeding. Objective: Report initial experience of hemospray use in treatment of upper GIT bleeding. Patients and Methods: The present study included twenty-five patients with acute GIT bleeding originating from variceal or non-variceal sources. Hemospray was endoscopically applied when active bleeding occurs; and outcome was measured. Results: success rate was achieved in 92.0% of cases with no unwanted side effects. Patients showed stable postoperative hemodynamics and needs no further intervention. Conclusion: hemospray appears to allow safe and effective control of upper gastrointestinal bleeding