Background: Hospital waste is one of the most common health related subject for health care provider. Effective surveillance of hospital waste as regard collection, storage, transportation and incineration in Al-Hussein or crushing and sterilization in Sayed Galal hospitals. The related health education paper is an important tool to increase the awareness of the health care providers and decrease the risk factors of developing blood born hepatitis among them. Objectives: Identifying the occupational health hazards to which the hospital waste workers were exposed in Al-Azhar University Hospitals, and find out the proportion of hospital waste workers who were infected by blood born hepatitis (B and C), and identifying risk factors of developing infection by blood born hepatitis (B and C). Subjects and Methods: Four hundred subjects from workers and nurses at different Departments of Al-Hussein and Sayed Galal University Hospitals as exposed group, and another 400 subjects as non exposed (control group) from security, and different administrative departments. Results: Fifty Six subjects developed hepatitis antibodies at the end of the study period, and 344 subjects were not infected. 52.5% were mainly injured by needle stick, 89.2% were mainly supervised by nurses, 32.5% did periodic medical examination, and 77.5% agreed that safety box easily opened, accessible and evacuated before filling, 90% were using the personal protective equipments and 37.5% were vaccinated against HBV. There were 46.6% attending and following monthly the health education seminars.There were 74.8% attending and following the training courses seminars. 96.25% were knew and fellow the color coding specification and separation. 40% of the studied group worked less than five years, 30% from five to ten years and 30% more than ten years .70% were satisfied with job. There were 22.5% of the studied group had excellent knowledge before health education, 25% of the studied group had good knowledge before health education, 27.5 % had fair knowledge before health education, and 25% had poor knowledge before health education and changed after health education to 40%, 36.2, 18.8 and 5.0 respectively. 23.8 % of exposed were hypertensive, but only 11.5% of none exposed were hypertensive. 24.3 % of exposed were diabetics, but only 10.8% of none exposed were diabetic, 16.5 % hade chronic bronchitis and 11.3 % of exposed were asthmatic. Conclusion: Fourteen   of exposed had positive hepatitis Ab. 91.1% had positive PCR for HCV and HBV. 15% were positive hepatitis in Al-Hussein, but only 11% in Sayed Galal. 74.8% were attending and following the training courses seminars. 96.25% knew and fellow the color coding specification and separation. 40% of the studied group worked less than five years, 30% from five to ten years and 30% more than ten years. 70% were satisfied with job.