59186

EFFECT OF ATORVASTATIN ON RENAL INFLAMMATORY CYTOKINES AND SERUM CYSTATIN-C IN TYPE-1 DIABETIC RATS

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Last updated: 04 Jan 2025

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Abstract

Background: Diabetic nephropathy is one of the major causes leading to end-stage renal failure. There are conflicting reports about the impact of treatment with statins on the diabetic nephropathy through their anti-inflammatory effect. Local cytokine levels and activity are of great value for monitoring of pathological events in a target tissue. So, cytokines measurements in tissue or peripheral circulation have been an important part of the process of defining their role in health and disease. Cystatin-C is an alternative serum biomarker that has been proposed for estimating renal function that can replace or supplement serum creatinine. Objective: Evaluation of the possible effect of atorvastatin treatment on the renal damage caused by type-1 diabetes mellitus in the terms of renal inflammatory cytokines and serum Cystatin-C. Materials and Methods: Thirty two male albino rats were divided into four equal groups, Group A: non-diabetic rats (negative controls), Group B: type-1 diabetic rats (positive controls), Group C: non-diabetic rats received oral atorvastatin for 4 weeks, and Group D: type-1 diabetic rats received oral atorvastatin for 4 weeks. At the end of the designated period, animals were sacrificed, kidneys and blood samples were collected; IL-1β, IL-6, IL-10 and prostaglandin E2 were determined in kidney homogenate, while Cystatin-C, creatinine and urea were assayed in serum. Results: IL-1β, IL-6, IL-10 and PGE2 in diabetic rat kidney significantly elevated above control. In addition, serum Cystatin-C significantly elevated in the diabetic rats. Treatment of diabetic rats with atorvastatin caused decreases in all determined cytokines as well as Cystatin-C to levels near control values. Conclusion: Atorvastatin has the potential for protecting diabetes-induced renal injury in terms of decreasing renal inflammatory cytokines and serum Cystatin-C. However, the possible protective effect of atorvastatin should be supported by clinical studies. Background: Diabetic nephropathy is one of the major causes leading to end-stage renal failure. There are conflicting reports about the impact of treatment with statins on the diabetic nephropathy through their anti-inflammatory effect. Local cytokine levels and activity are of great value for monitoring of pathological events in a target tissue. So, cytokines measurements in tissue or peripheral circulation have been an important part of the process of defining their role in health and disease. Cystatin-C is an alternative serum biomarker that has been proposed for estimating renal function that can replace or supplement serum creatinine. Objective: Evaluation of the possible effect of atorvastatin treatment on the renal damage caused by type-1 diabetes mellitus in the terms of renal inflammatory cytokines and serum Cystatin-C. Materials and Methods: Thirty two male albino rats were divided into four equal groups, Group A: non-diabetic rats (negative controls), Group B: type-1 diabetic rats (positive controls), Group C: non-diabetic rats received oral atorvastatin for 4 weeks, and Group D: type-1 diabetic rats received oral atorvastatin for 4 weeks. At the end of the designated period, animals were sacrificed, kidneys and blood samples were collected; IL-1β, IL-6, IL-10 and prostaglandin E2 were determined in kidney homogenate, while Cystatin-C, creatinine and urea were assayed in serum. Results: IL-1β, IL-6, IL-10 and PGE2 in diabetic rat kidney significantly elevated above control. In addition, serum Cystatin-C significantly elevated in the diabetic rats. Treatment of diabetic rats with atorvastatin caused decreases in all determined cytokines as well as Cystatin-C to levels near control values. Conclusion: Atorvastatin has the potential for protecting diabetes-induced renal injury in terms of decreasing renal inflammatory cytokines and serum Cystatin-C. However, the possible protective effect of atorvastatin should be supported by clinical studies.

DOI

10.12816/0029132

Keywords

Atorvastatin, Diabetes mellitus, Cystatin-C, Interleukins

Authors

First Name

Yasser

Last Name

I. Kandil

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Affiliation

Biochemistry Department, Faculty of Pharmacy, Al-Azhar University. Cairo, Egypt

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First Name

Emad

Last Name

A. El-Bassiouni

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Affiliation

Pharmacology Department, Medical Research Institute, Alexandria University, Egypt

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Volume

45

Article Issue

2

Related Issue

8926

Issue Date

2016-04-01

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2016-04-01

Publish Date

2016-04-01

Page Start

319

Page End

330

Print ISSN

1110-0400

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https://amj.journals.ekb.eg/article_59186.html

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https://amj.journals.ekb.eg/service?article_code=59186

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11

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Original Article

Type Code

941

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Journal

Publication Title

Al-Azhar Medical Journal

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https://amj.journals.ekb.eg/

MainTitle

EFFECT OF ATORVASTATIN ON RENAL INFLAMMATORY CYTOKINES AND SERUM CYSTATIN-C IN TYPE-1 DIABETIC RATS

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Article

Created At

22 Jan 2023