Background: Exercise, which is the keystone of type 2 diabetes millets (T2DM) and obesity treatment, has revealed a great variance of effects on adipokines levels. Vaspin is a new adipocytokine with insulin-sensitizing effects, but its regulation in obesity and physical exercise are not clear yet. Objective: Exploring the effect of continuous and intermittent exercise on circulating levels of vaspin and its relation to some metabolic changes and inflammatory markers in lean and obese rats. Material and methods: A total number of60 adult male local strain albino rats were used. Rats were divided into 2 main groups: group I" lean group, and group II "HFD group; Each group was further subdivided into 3 equal subgroups: group I A; control group with sedentary life, group I B; rats were exposed to continuous exercise, and group I C; rats were exposed to intermittent exercise. Serum vaspin, insulin, glucose, triglycerides, cholesterol, HDL, TNF and CRP levels were measured. In addition, BMI, HOMA-IR and atherogenic index were calculated in all groups. Results: Serum vaspin levels were higher in obese (HFD) than in lean rats. Vaspin levels  increased in continuous and intermittent exercised lean groups, and decreased in HFD induced obesity. Vaspin correlated negatively with inflammatory markers, HOMA-IR, TC TG and positively with HDL-C. Conclusion: Serumvaspin levels were up regulated in HFD induced obesity. Intermittent exercise was more effective than continuous exercise in improving metabolic parameters and ameliorating inflammatory effect of obesity partially by modulating circulating vaspin levels in lean and obese rats.