Background: The rising rates of diabetes mellitus indicate a need for enhanced education and training in effectively preventing, screening, diagnosing and treating the condition. Objective: Determination of the possible beneficial effects of Nigella Sativa Seeds powder in treatment of diabetes mellitus. Material and Methods:  One hundred and forty adult male albino rats of local strain, 8 weeks of age and weighing from 120 to 150 g, were chosen as an animal model for this study.  The animals were divided into control and diabetic groups.  Diabetes was induced by alloxan. The experiment continued for 60 days of Nigella Sativa seeds powder treatment. .  After12 hours over night fasting at the end of experiment, morning blood samples and liver specimens were collected for the determination of lipid profile, aspartate aminotransferase “AST", alanine amino transferase “ALT", creatinine, urea and liver architecture. Results: Lipid profile, AST, ALT and liver structure showed significant improvement with NS powder treatment. No statistically significant differences occur in creatinine or urea. It caused a sort of protection to the hepatocytes from the effect of diabetes mellitus. Conclusion:  Oral administration of NS seeds did not give any toxic effects on liver function evaluating hepatic enzymes level as well as histopathological changes of liver tissue.