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110680

Gene Xpert MTB/RIF Assay as A New Tool for Tuberculosis Diagnosis and Detection of Rifampin Resistance

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Last updated: 22 Jan 2023

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Abstract

Background: Mycobacterium tuberculosis (MTB) is one of the top ten causes of death and the leading cause of a single infectious agent (above HIV/AIDS) Worldwide. Each year millions of people continue to fall sick with TB. Aim: To evaluate the MTB/RIF assay for rapid diagnosis and detection of rifampin resistance of tuberculosis in both positive and negative smears-as well as pulmonary and non-pulmonary clinical specimens. Patients and Methods: A prospective study was conducted on 50 patients (37 pulmonary, 13 extrapulmonary) patients attending the Outpatient Clinic and inpatients of Chest Department, Benha University Hospital, Egypt. Pulmonary samples were analyzed by (Sputum smear exam. by Ziehl Neelsen (ZN) stain, culture on Lowenstein-Jensen(LJ)media, and assessment of rifampin sensitivity). Extrapulmonary samples were analyzed by reference methods (pathological diagnosis) and by GeneXpert. Results: pulmonary patients' smears were 32 (86.5%) positive and 5 (13.5%) negative. Culture on LJrevealed rifampin sensitivity in 33/37 (89.1%) and rifampin-resistant in 4/37 (10.9%), GeneXpert was positive in 32(86.5%) and negative in 5 (13.5%), rifampin assay was sensitive in 33 (89.1%) and resistant in 4(10.9%). All extrapulmonary patients, (n=13) were positive on GeneXpert and sensitive for rifampin. Conclusion: GeneXpert could be considered as a leading way to early diagnosis, treatment, and prevention of transmission of tuberculosis which could reduce TB- associated morbidity and mortality.

DOI

10.21608/scumj.2019.110680

Keywords

Ziehl-Neelsen Smears, Lowenstein-Jensen media, and Rifampin sensitivity

Authors

First Name

Rabab

Last Name

Al Olimey

MiddleName

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Affiliation

Department of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, Egypt

Email

rababsalah76@gmail.com

City

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Orcid

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First Name

Sohier

Last Name

AbdElSamie

MiddleName

-

Affiliation

Department of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, Egypt

Email

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City

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Orcid

-

First Name

Mohamed

Last Name

Kamel

MiddleName

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Affiliation

Department of Internal Medicine (Chest), Faculty of Medicine, Benha University, Egypt

Email

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City

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Orcid

-

First Name

Afaf

Last Name

Khamis

MiddleName

-

Affiliation

Department of Chemical and Clinical Pathology, Faculty of Medicine, Benha University, Egypt

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Volume

22

Article Issue

2

Related Issue

14304

Issue Date

2019-10-01

Receive Date

2020-09-02

Publish Date

2019-10-01

Page Start

179

Page End

186

Print ISSN

1110-6999

Online ISSN

2090-2581

Article File

SCUMJ_Volume 22_Issue 2_Pages 179-186.pdf

PDF . 269.1kB

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https://scumj.journals.ekb.eg/article_110680.html

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https://scumj.journals.ekb.eg/service?article_code=110680

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Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

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https://scumj.journals.ekb.eg/

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Article

Created At

22 Jan 2023