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57473

The Effect of Smear Layer Removal on Internal Apical Baterial Leakage along Root Canal Fillings Using Three Diferent Obturation Techniques

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Last updated: 22 Jan 2023

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Abstract

Aim: This study was conducted to evaluate the influence of smear layer removal on internal apical bacterial leakage of infected root canals obturated with 3 techniques; single cone, lateral compaction and vertical compaction with System-B. Materials and Methods: A total of 140 human teeth were prepared using ProTaper rotary files. The samples were classified into 2 equal groups according to whether the smear layer was removed or not. The root canals were inoculated with Enterococcus faecalis suspension and incubated at 37°C for 1 week.Each group of roots was then subdivided into 3 equal experimental subgroups according to the obturation technique. Following obturation, the samples were suspended in Trypticase Soy Broth (TSB) and the broth was checked on daily basis for turbidity, as an evidence for internal apical bacterial leakage up to 3 months. Results: All obturation techniques leaked more slowly in the absence of smear layer than in its presence, the difference was statistically significant for single cone technique, whereas for lateral compaction and vertical compaction techniques the difference was insignificant. It was also observed that smear layer removal significantly minimized the counts of leaked bacteria through the apical foramen. Conclusions: Smear layer removal minimizes the counts of leaked bacteria through the previously infected root canals and may enhance the sealability of the root canal obturation.  

DOI

10.21608/scumj.2011.57473

Keywords

apical leakage, Smear layer, E. faecalis, Spectrophotometer

Authors

First Name

Walaa

Last Name

Gharieb

MiddleName

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Affiliation

Department of Endodontics, Faculty of Dentistry, Suez Canal University, Egypt

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First Name

Naguib

Last Name

Aboul-Enein

MiddleName

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Affiliation

Department of Endodontics, Faculty of Dentistry, Suez Canal University, Egypt

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Orcid

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First Name

Hassan

Last Name

Nasr El-Islam

MiddleName

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Affiliation

Department of Microbiology and Immunology, Faculty of Medicine, Suez Canal University, Egypt

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Volume

14

Article Issue

1

Related Issue

7461

Issue Date

2011-03-01

Receive Date

2019-11-07

Publish Date

2011-03-01

Page Start

31

Page End

36

Print ISSN

1110-6999

Online ISSN

2090-2581

Link

https://scumj.journals.ekb.eg/article_57473.html

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https://scumj.journals.ekb.eg/service?article_code=57473

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6

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

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Article

Created At

22 Jan 2023