Background: Female sexual dysfunction is a condition that describes patients with low libido, problems with sexual arousal, inability to achieve orgasm, and dyspareunia. Although not consistent, studies have found that sexual complaints are common in women with pelvic floor disorders. Aim: The aim of this study was to evaluate the impact of vaginal wall prolapse on the female sexual function (FSF). Material and Methods: The study was conducted at Suez Canal University Hospital, Ismailia at outpatient clinic from October 2012 to April 2013. Sixty patients with vaginal wall prolapse were selected randomly. Data on female sexual function and satisfaction were obtained by female sexual function index questionnaire from 60 patients with vaginal prolapse and 60 controls women. The six domains of the female sexual function questionnaire (desire, arousal, lubrication, orgasm, satisfaction, and pain) were compared between both groups. A score of 26.55 was defined as the optimal cut score between normal and pathological values. Results: The means of the six domains of the Female Sexual Function Index (FSFI) were significantly different between both groups (p <0.0001).There was a significant difference in the mean of the total score between patient and control group (20.92, and 28.03 respectively) taking in consideration that all women in the patient group had vaginal wall prolapse. Conclusion: Vaginal wall prolapse has a major poor effect on the female sexual function.