Background: The World Health Organization describes diabetes mellitus as the most common endocrine disease in the world. Diabetes mellitus is the most common cause of neuropathy worldwide. About 25–50% of diabetic patients in developed countries have peripheral polyneuropathy. Prolonged B12 deficiency can lead to nerve degeneration and irreversible neurological damage. Aim: This study was conducted aiming at assessing the prevalence of peripheral neuropathy among type 2 diabetic patients attending Suez Canal University Outpatient clinics. A secondary objective of the study was to identify the relation between vitamin B and diabetic neuropathy. Patients and Methods: A convenience sample was used in this cross sectional study, which was conducted in Suez Canal University Outpatient clinics. A total of 362 type 2 diabetic patients, responded to questionnaire and screening examination, using the Michigan Neuropathy Screening Instrument (MNSI). Results: The present study showed that the mean age was 55.12 ± 9.26 and about 81.2% of the study population were females. More than, two-thirds (69.1%) were married. In addition, about 37%, had diabetes for over 10 years. The study revealed that 34% of the study population had peripheral neuropathy according to Michigan score results. The most frequent symptom was numbness (83.4 %). Vibration perception was absent in half of patients. Nearly monofilament was absent in one-third (34.5%) of patients. As regards vitamin B12 supplements, the results revealed that about 87.1% of neuropathic patients had taken vitamin B12. Conclusion: The result of the present study concluded that peripheral neuropathy is a very common problem in diabetic patients, which accounts 34% in our study.