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46882

Effect of Bone Marrow-Derived Stem Cells on the Submandibular Salivary Glands of Streptozotocin-induced Diabetic Rats

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Last updated: 22 Jan 2023

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Abstract

Background: Stem cells are one of the most fascinating areas of biology today they play an important role in our bodies from embryonic development through adulthood, and diabetes mellitus (DM) is one of the most common degenerative diseases that affect all body tissues and organs. Objectives: The aim of this study was to evaluate the effect of bone marrow derived stem cells on the submandibular salivary glands in diabetic rats. Materials and methods A Total of sixty, adult male albino rats with body weight ranging from 180-200 grams were used in the present investigation. They were divided as follows, Group 1 (15 animals) served as controls. Group 2 (15 animals), they were subjected to a single intraperitoneal injection of streptozotocin. Group 3: consisted of 30 animals, they were subdivided as follows: Subgroup 3.1 (15 animals), they were used for isolation and culture of bone marrow stem cells and Subgroup 3.2: (15 animals)they were subjected to a single intraperitoneal injection of streptozotocin, the same as group 2, in a dose of 60 mg/kg body weight for induction of diabetes mellitus. One week later, they were subjected to a single intravenous infusion of mesenchymal bone marrow stem cells. At the end of the experiment the animals of the different groups were sacrificed by cervical dislocation, their submandibular salivary glands dissected out, separated into right and left glands for histological and ultrastructural examination. Results Light and transmission electron microscopic results showed the degenerative changes of diabetes in group 2 and the high regenerative changes of bone marrow derived stem cells in group 3.2 compared to the control group 1. Conclusion Stem cells have magnificent regenerative effect in diseased and injured tissues in different organs.

DOI

10.21608/scumj.2017.46882

Keywords

Parenchymal elements, Embryonic development, adulthood, Transmission electron microscope

Authors

First Name

Waleed

Last Name

Shaker

MiddleName

M.

Affiliation

Department of Oral Biology, Faculty of Dentistry, South Valley University

Email

dr_waleed@dent.svu.edu.eg

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Orcid

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First Name

Rasha

Last Name

Taha

MiddleName

M.

Affiliation

Department of Oral Biology, Faculty of Dentistry, Suez Canal University, Egypt

Email

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City

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Orcid

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First Name

Laila

Last Name

Ghali

MiddleName

S.

Affiliation

Department of Oral Biology, Faculty of Oral and Dental Medicine, Cairo University, Egypt

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Orcid

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Volume

20

Article Issue

1

Related Issue

6811

Issue Date

2017-03-01

Receive Date

2019-09-02

Publish Date

2017-03-01

Page Start

29

Page End

37

Print ISSN

1110-6999

Online ISSN

2090-2581

Link

https://scumj.journals.ekb.eg/article_46882.html

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https://scumj.journals.ekb.eg/service?article_code=46882

Order

4

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

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Article

Created At

22 Jan 2023