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65514

Natural Killer T cells in Egyptian Patients with HCV-associated Non-alcoholic Fatty Liver Disease

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Last updated: 22 Jan 2023

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Abstract

Background:Studies on Natural Killer T (NKT) cells in Non-alcoholic fatty liver disease (NAFLD) have shown contradictory results. Moreover, the effect of HCV superinfection on NKT cells in NAFLD patients have not been previously studied. Aim: to assess the effect of the concomitant HCV infection on the frequency of NKT cells and CD8+ T cells in a cohort of Egyptian patients with hepatic steatosis. Subjects and Methods: Two-hundred subjects were divided into four equal groups, 1) concomitant hepatic steatosis/HCV, 2) HCV (without steatosis), 3) steatosis (without HCV), and 4) normal controls. NKT and CD8+ T cells were identified by monoclonal antibodies surface staining for (CD3, CD56, CD161), and (CD3, CD8) respectively using flow cytometry. Results: CD3+/CD56+/CD161+ NKT cells were significantly higher in patients with either steatosis (alone) or concomitant HCV/steatosis compared to control (p<0.0001 each), while comparable NKT frequency was found between HCV group and control (p=0.72). A significant increase in CD3+/CD8+ T cells was noted in the three patients' groups compared to control (p=0.0001, 0.002 and 0.001, respectively). Interestingly, NKT cells were significantly higher in patients with steatosis/HCV compared to those with HCV alone (p<0.0001), while, they did not differ betweenconcomitant HCV/steatosis and steatosis (alone) group. Higher frequencies of both NKT cells and CD8+ T cells were significantly associated with increased severity of steatosis. In HCV group, NKT frequencies were unrelated to steatosis severity. Conclusion: NKT cells are significantly elevated in patients with hepatic steatosis irrespective to the presence or absence of concomitant HCV infection.  

DOI

10.21608/scumj.2019.65514

Keywords

NKT, CD8+ T, steatosis, Egypt, HCV

Authors

First Name

Raghda

Last Name

Ali

MiddleName

K

Affiliation

Department of Clinical Pathology, Ismailia Oncology Hospital, Egypt

Email

-

City

-

Orcid

-

First Name

Soha

Last Name

Younes

MiddleName

E

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Adel

Last Name

Hassan

MiddleName

A

Affiliation

Department Tropical Medicine, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

Hassan

MiddleName

M

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt

Email

-

City

-

Orcid

-

First Name

Amro

Last Name

El-Sakka

MiddleName

A

Affiliation

Fifth year Medical Student, Faculty of Medicine, Suez Canal University, Egypt

Email

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City

-

Orcid

-

First Name

Rasha

Last Name

Emad

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Port Said University, Egypt

Email

-

City

-

Orcid

-

First Name

Howayda

Last Name

Hassoba

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Suez Canal University, Egypt

Email

hhassoba@yahoo.com

City

-

Orcid

0000-0001-9619-1953

Volume

22

Article Issue

1

Related Issue

6743

Issue Date

2019-03-01

Receive Date

2019-12-18

Publish Date

2019-03-01

Page Start

95

Page End

105

Print ISSN

1110-6999

Online ISSN

2090-2581

Article File

SCUMJ_Volume 22_Issue 1_Pages 95-105.pdf

PDF . 735.8kB

Link

https://scumj.journals.ekb.eg/article_65514.html

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https://scumj.journals.ekb.eg/service?article_code=65514

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12

Type

Original Article

Type Code

938

Publication Type

Journal

Publication Title

Suez Canal University Medical Journal

Publication Link

https://scumj.journals.ekb.eg/

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Article

Created At

22 Jan 2023