Introduction: Female sexual dysfunction (FSD) is an underestimated common problem with serious effects on women's quality of life. Female sexual function changes considerably during pregnancy and the postpartum period. During this time, the perineum and pelvic floor muscles are damaged. Type of delivery and perineal trauma during childbirth is associated with sexual problems after childbirth. There is controversy over the effect of mode of delivery on female sexual function. Aim: The aim of this study was to assess the relationship between mode of delivery and postpartum female sexual function. Materials and Methods: This was a prospective, descriptive study. 42 women (age range from 18–35years) were recruited in this cross-sectional study. Females were divided into two groups according to their mode of delivery, including: group A, normal labour (NL) (group NL, N = 21); group B, cesarean section (C/S) (group C/S, N = 21). Sexual function was assessed using Female Sexual Function Index (FSFI) and Depression, Anxiety, and stress scale )DASS-21(. Results: There were statistically significant decrease of desire, arousal, orgasm and total score of FSFI and while there was significant increase in lubrication and satisfaction domains in NVD group when compared to CS group. The study showed that, there was significant decrease in depression, anxiety and stress in NVD group when compared to CS group according to DASS-21 score. Conclusions: Mode of delivery had actually affected female sexual function in postpartum period.