The present study aimed to utilize the rennet coagulation test (RCT) as a simple, applicable and indicator (as innovated method) for detecting the subclinical mastitis in the buffalos and cows farms by using the value of the calculated rennin units (RU /ml rennet). Moreover, chemical composition of milk as well as curd tension, curd synersis and yield of milk were done. Results of RCT were compared with the traditional methods used to detect the subclinical mastitis. Results showed no significant differences between the RCT method and the other methods of detecting mastitis milk. There were a decrease in the total solids content of the milk, the strength of the resulting curd, and the yield of mastitic milks while curd synersis, chloride, sodium and pH values were increased.