The current study was conducted at agro-eycosystem of Basra province during the period 2013-2014, to evaluate the diversity of spiders community. 24 species, 23 genera belong to 10 families was recorded, the most relative abundance was registered for family Lycosidae 0.80 . the most divers month was November 2.88, the results of correlation revealed a negative correlation between number of spiders and wind speed, as well as air temperature degree, whereas the number of spiders correlated positively with rain quantity and relative humidity.