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67988

Organogenesis of Tomato (Lycopersicon esculentum Mill.) cv. Sandra and Rocky by Hypocotyl, Cotyledon and Leaf Explants

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Last updated: 04 Jan 2025

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Abstract

         In vitro regeneration protocols were established for two Tomato (Lycopersicon esculentum Mill.) cultivars (Sandra and Rocky) grown in Kurdistan region using hypocotyl, cotyledonous leaves and leaf explants. The explants excised from 12-15 days old of in vitro plantlets cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators. High organogenesis percentage (100%) was achieved from hypocotyl grown in MS medium augmented with 1.0mgl-1 BA+ 0.5mgl-1 kinetin, which record approximately 3.75 in Sandra and 4.09 in Rocky cultivar as average number of shoots produced from each explant. While the highest organogenesis percentage (100%) was formed from cotyledon leaves using 1.5 mgl-1 BA+0.5mgl-1 kinetin that gave around 3.5 in Sandra cultivar as average number of shoots / explant. In contrast, the highest organogenesis was found on MS medium supplemented with 2.0mgl-1 BA+1.0 mgl-1 kinetin and the average number of shoots produced from each explant were approximately 3.17 in Rocky cultivar. The results of combination between 5.0mgl-1 BA with (0.0, 0.2, 0.5 and 1.0)mgl-1 NAA, indicated that the highest organogenesis percentage was recorded from hypocotyl (91.67 % with 6.5 shoots/explant and 66.67% with 4.0 shoots/ explant) in Sandra and Rocky cultivars respectively grown on MS medium  supplemented with 5.0 mgl-1 BA alone. While the highest organogenesis percentage (100%) was induced from cotyledonous leaves in both cultivars (3.67 shoots for Sandra and 3.08 for Rocky) on MS medium supplemented with 5.0mgl-1 BA +0.2mgl-1 NAA. Meanwhile, the highest organogenesis percentage was obtained from leaf explants for both cultivars on the combination of 1.0mgl-1 BA +0.5mgl-1 kinetin, (77.78% and 66.67%) in Sandra and Rocky cultivars respectively. The regenerated plants were successfully transplanted to the field for acclimatization
 
 

DOI

10.21608/asajs.2020.67988

Keywords

sLycopersicon esculentum, micropropagation, Organogenesis, TDZ, kinetin

Authors

First Name

Gharbia Hormiz

Last Name

Danial

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Affiliation

Scientific Research Center (SRC), College of Science, University of Duhok, Duhok, IRAQ

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City

دهوک

Orcid

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First Name

Diaa Ayoub

Last Name

Ibrahim

MiddleName

-

Affiliation

Scientific Research Center (SRC), College of Science, University of Duhok, Duhok, IRAQ

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Volume

3

Article Issue

5

Related Issue

10273

Issue Date

2020-01-01

Receive Date

2020-01-12

Publish Date

2020-01-01

Page Start

31

Page End

54

Print ISSN

2537-0804

Online ISSN

2537-0855

Link

https://asajs.journals.ekb.eg/article_67988.html

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https://asajs.journals.ekb.eg/service?article_code=67988

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2

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المقالة الأصلية

Type Code

937

Publication Type

Journal

Publication Title

المجلة العربية للعلوم الزراعية

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https://asajs.journals.ekb.eg/

MainTitle

Organogenesis of Tomato (Lycopersicon esculentum Mill.) cv. Sandra and Rocky by Hypocotyl, Cotyledon and Leaf Explants

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Article

Created At

22 Jan 2023