2-bis(imidazole-2-yl )methylene ) thiosemicarbazone and its complexes with Pd (II), Cu (II), Hg (II), and Cd (II),ions have been prepared and characterized by elemental analysis, molar conductivity, spectroscopy (IR, uv. VIS, 1H NMR, mass spectrometry) and magnetic moment. The thiosemicarbazone coordinates to the metal ions via sulfur and azomethine nitrogen atom. The free ligand and its metal complexes have been tested in vitro against a number of microorganisms, to assess their antimicrobial properties. Antibacterial  screening of the ligand and its complexes  was conducted against Streptococcus pneumonia (G+), Bacillis subtilis (G+), Pseudomonas aeruginosa (G-), Escherichia coli (G-), also antifungal activity was examined against Aspergillus fumigates and Candida albicans. The experimental results show that nearly some complexes exhibit antibacterial activity highest than the free ligand but less than the standard..