Bioplastic products represent one of the most important eco-friendly alternatives to petrochemical plastics. Therefore, the study aims at producing polyhydroxyalkanoates (PHAs) using industrial wastes by the most potent bacterial isolate. Seventy-eight isolates were obtained from different localities in Arab Dairy Products Company (Panda, Qalyubia-Egypt). These isolates were subjected to qualitative and quantitative screening tests. Out of these isolates, bacterial isolate BZU-B2 showed the highest production of PHA. The selected strain was identified biochemically as Bacillus cereus by biochemical characterization. Nutritional and physiological factors that influence PHA productions were optimized. Temperature 37 ͦC, whey concentration of 50%, and ammonium chloride 1.5g L were chosen as the best factors in achieving the highest production of PHA 1.63 g/L with a recovery yield of 33.42 (%, w/w) after 96h. The characteristics of extracted PHA were analyzed using FTIR-spectroscopy techniques. This study focused on improving the ability of Bacillus cereus to give a highly purified yield of PHA from whey (a low-cost carbon source).