Many Streptomyces species are effective as biological controls for many pathogenic fungi. The research on new biocontrol principles for some phytopathogenic species that aren't as effective as traditional fungicides have sparked global interest. The Streptomyces genus is responsible for most of all known antibiotics. These are used in various fields such as medical and agricultural settings. Streptomyces was isolated from soils obtained from different sites in Egypt. Antifungal activity against phytopathogenic fungi was tested on 65 isolates, i.e., Alternaria sesame, Fusarium oxysporum, and Rhizoctonia solani. The Streptomyces isolate BF26 showed the highest antifungal activity against the investigated fungi by dual culture bioassay and paper disc diffusion techniques. Biochemical, microscopy, and morphological procedures were used to characterise them better. The results showed that the isolate BF26 was gram-positive and belonged to members of the Streptomyces genus. Based on DNA sequences using 16S rRNA, the data showed that the isolate BF26 belonged to Streptomyces bungoensis. The isolate BF26 was saved in the NCBI Gene nucleotide sequence database with the accession numbers ON130197.